PPDB

 The Plant Proteome Database

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AT1G32440.1   plastidial pyruvate kinase 3
Lab Annot. pyruvate kinase (typically homotetramer)
Mapman: 11.1.30 lipid metabolism.FA synthesis and FA elongation.pyruvate kinase
Curated Location plastid stroma (Pubmed: 23198870 )
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT5G52920.1 11Arabidopsis thaliana04717586486 (279)C
AT3G22960.1 11Arabidopsis thaliana5E-1234824462687 (421)C
AT5G08570.1 11Arabidopsis thaliana2E-864573956100 (58)_
Os10g42100.1 7Oryza sativa0492768631 (31)C
Os01g47080.1 7Oryza sativa0481768531 (31)C
Os07g08340.1 7Oryza sativa2E-123488436139 (39)C
GRMZM2G033526_P02 3.21Zea mays0488758661 (12)C
v1_GRMZM2G033526_P03 4a53Zea mays0488758614 (14)C
Zm00001eb390590_P001 agpv5Zea mays051072830 (0)C
Show all       

#Spots: The number of publicly accessible spots are in parenthesis
Prediction
PFAM: Cellulase(1) HpcH_HpaI(2) IMPDH(2) PK(1) PK_C(1)
TargetP: Chloroplast (Class 2 C0.930; M0.065; S0.003; _0.164)
Predotar:
Subcel. Location: Stroma
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 571 aa (-cTP 516)
Molecular Weight 62.61 kDA(-cTP 56.87)
PI 7.68(-cTP 6.41)

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  
Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
94Detail       root [B. rapa] plastid stroma 
303Detail       leaf [A. thaliana] chloroplast stromawild-type
412Detail       leaf [A. thaliana] stroma - R-X102 tag-CNPAGE clpr2-1-TapTagR2
544Detail       leaf [A. thaliana] stroma (FPLC) <600 kDa (LMA) wild-type
871Detail       leaf [A. thaliana] chloroplast stromawild-type
1202Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1207Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1208Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1399Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1482Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 1)
1484Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 2)
1485Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 3)
1486Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 3)
1937Detail       leaf [A. thaliana] chloroplast Prep1_Prep2
1942Detail       leaf [A. thaliana] stroma WT stromawild-type
1943Detail       leaf [A. thaliana] stroma WT stromawild-type
1949Detail       leaf [A. thaliana] stroma ClpS stromaclpS
2009Detail       leaf [A. thaliana] total leaf tissue wild-type
2249Detail       seedlings [A. thaliana] total tissue gfs9
2252Detail       seedlings [A. thaliana] total tissue gfs9
2253Detail       seedlings [A. thaliana] total tissue atg5
2289Detail        [A. thaliana] Clp C1 TRAP
2302Detail        [A. thaliana] Clp C1 WT

* For details about the exprimental sources click here.

Published Proteomics Data

 15052571(soluble leaf)
18431481(chloroplast)
19546170(mature pollen grains)
19995723(chloroplast reference proteome)
21166475(cytosolic-fractions-crude)
21173025(root proteome)

Comparative Proteomics Data

©Klaas J. van Wijk Lab, Cornell University   Web Accessibility Help