PPDB

 The Plant Proteome Database

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AT1G22300.3   general regulatory factor 10
Lab Annot. 14-3-3 protein GF14 epsilon (grf10)
Mapman: 30.7 signalling.14-3-3 proteins
Curated Location not plastid (supported by in-house exprements)

TAIR curated location: plasma membrane & chloroplast stroma & cytoplasm9276953
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT1G22300.2 11Arabidopsis thaliana2E-176240100100461 (215)_
AT1G22300.1 11Arabidopsis thaliana2E-176240100100467 (216)_
AT1G34760.2 11Arabidopsis thaliana2E-143241809229 (5)_
Os08g33370.1 7Oryza sativa1E-125239728668 (68)_
Os08g33370.2 7Oryza sativa1E-125239728668 (68)_
Os03g50290.2 7Oryza sativa7E-123242728377 (77)_
Zm00001eb179490_P001 agpv5Zea mays9E-12323971850 (0)_
Zm00001eb179490_P002 agpv5Zea mays9E-12323971850 (0)_
v1_GRMZM2G091155_P02 4a53Zea mays9E-1232397185158 (156)_
Show all       

#Spots: The number of publicly accessible spots are in parenthesis
Prediction
PFAM: 14-3-3(1) Ca_bind_SSO6904(1) DUF4710(1) HBM(3) Protoglobin(2) Sec8_exocyst(3) TP
TargetP: Cytoplasm (Class 2 C0.067; M0.182; S0.069; _0.915)
Predotar:
Subcel. Location:
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 251 aa (-cTP 225)
Molecular Weight 28.57 kDA(-cTP )
PI 4.85(-cTP )

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  
Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
451Detail       leaf [A. thaliana] total leaf tissue clpr4-1
528Detail       leaf [A. thaliana] total leaf tissue wild-type (wt2)
621Detail       leaf [A. thaliana] total leaf tissue clpr4-1
622Detail       leaf [A. thaliana] total leaf tissue wild-type
1018Detail        [aphid]  
1125Detail       leaf [A. thaliana] chloroplast plastoglobulek2k3
1346Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 2)
1347Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 3)
1348Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 1)
1349Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 2)
1394Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1399Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1402Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1404Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1413Detail       leaf [A. thaliana] total leaf tissue -114 rep1tR1 wild-type
1415Detail       leaf [A. thaliana] total leaf tissue 1.14 rep 1.1 clpr2-1
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1421Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1422Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1423Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 3)
1424Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 1)
1425Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 2)
1426Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 3)
1445Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 1)
1450Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 3)
1483Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 2)
1519Detail       leaf [A. thaliana] wild-type
1522Detail       leaf [A. thaliana] wild-type
1523Detail       leaf [A. thaliana] wild-type
1531Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP wild-type (rep. 1)
1540Detail       leaf [A. thaliana] Thylakoids plastoglobulewild-type
1541Detail       leaf [A. thaliana] Thylakoids plastoglobuleM-48
1542Detail       leaf [A. thaliana] wild-type
1613Detail       Natural Senescence leaf [A. thaliana]  
1765Detail       leaf [A. thaliana] Thylakoids thylakoid membraneCol-0
1766Detail       leaf [A. thaliana] Thylakoids thylakoid membraneMet1-1
1828Detail       leaf [A. thaliana] Thylakoids thylakoid membraneCol-0 Fluctuating light
1829Detail       leaf [A. thaliana] Thylakoids thylakoid membraneMet1-1 Fluctuating light
1875Detail       Total leaf [A. thaliana] total soluble wild-type
1930Detail       leaf [A. thaliana] total leaf tissue Col_0 Wild-type
1931Detail       leaf [A. thaliana] total leaf tissue DXS_Flag
1942Detail       leaf [A. thaliana] stroma WT stromawild-type
1943Detail       leaf [A. thaliana] stroma WT stromawild-type
1944Detail       leaf [A. thaliana] stroma UVR stromaUVR
1947Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1948Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1949Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1999Detail       leaf [A. thaliana] stroma ClpS stromaclpS1
2000Detail       leaf [A. thaliana] stroma ClpS stromaclpS1
2001Detail       leaf [A. thaliana] stroma ClpS stromaclpS1
2002Detail       leaf [A. thaliana] stroma ClpS stromaclpS1
2013Detail       leaf [A. thaliana] total leaf tissue wt
2028Detail       leaf [A. thaliana] WT PG plastoglobuleWT
2029Detail       leaf [A. thaliana] over expressed M48 plastoglobuleM48
2030Detail       leaf [A. thaliana] over expressed M48 plastoglobuleM48
2160Detail       leaf [A. thaliana] stroma Clp stromaP3-strepII 13
2192Detail        [A. thaliana] total soluble P3-trap-strepII 16
2193Detail        [A. thaliana] total soluble P3-strepII 26
2214Detail       leaf [A. thaliana] total leaf tissue Clp P5 st
2215Detail       leaf [A. thaliana] total leaf tissue Clp P5 trap st
2217Detail       leaf [A. thaliana] total leaf tissue Clp P5 trap st
2218Detail       Total soluble [A. thaliana] Total soluble proteins cGEP strep
2219Detail       Total soluble [A. thaliana] Total soluble proteins cGEP strep
2222Detail       Total soluble [A. thaliana] Total soluble proteins cGEP strep
2223Detail       Total soluble [A. thaliana] Total soluble proteins cGEP strep
2224Detail       leaf [A. thaliana] total leaf tissue Clp P3 strep
2225Detail       leaf [A. thaliana] total leaf tissue Clp P3 trap
2226Detail       leaf [A. thaliana] total leaf tissue wild-type
2245Detail       seedlings [A. thaliana] total tissue wild-type
2246Detail       seedlings [A. thaliana] total tissue gfs9
2247Detail       seedlings [A. thaliana] total tissue atg5
2248Detail       seedlings [A. thaliana] total tissue wild-type
2249Detail       seedlings [A. thaliana] total tissue gfs9
2250Detail       seedlings [A. thaliana] total tissue atg5
2251Detail       seedlings [A. thaliana] total tissue wild-type
2252Detail       seedlings [A. thaliana] total tissue gfs9
2253Detail       seedlings [A. thaliana] total tissue atg5
2266Detail       leaf [A. thaliana] stroma Clp Clp C1 wild-type
2267Detail       leaf [A. thaliana] stroma Clp Clp C1 TRAP
2296Detail        [A. thaliana] Clp C1 WT
2297Detail        [A. thaliana] Clp C1 WT
2302Detail        [A. thaliana] Clp C1 WT
2306Detail        [A. thaliana] Clp C1 TRAP
2307Detail        [A. thaliana] Clp C1 TRAP
2399Detail       leaf-soluble [A. thaliana] GFP-nano-trap wt/DUF760-1GFP/ClpC1WTSTREP

* For details about the exprimental sources click here.

Published Proteomics Data

 16358359(cell suspension (Gamborg))
16619310(14-3-3 proteins)
17317660(Plasma Membrane proteome)
18538804(total leaf)
17407188(cotyledon)
17828791(total leaf)
17293592(total seedlings û light and dark grown)
17137349(ATP-binding-mitochondria cell suspension)
18463617(suspension cells-phosphorylated proteins)
19452453(14-3-3-interacting proteins)
18814325(cotyledons)
19200160(flower-stage 12)
20118269(developing seeds)
19036721(detergent resistant plasma membrane (DRM) proteins (Cell cultures))
19525416(leaf (wt and clpr4-1 mutant))
19334764(plasma membrane (cell culture))
19714877(interactomes 14-3-3 complexes (cell culture))
19888209(80S polysomal fraction)
19888209(leaves)
19329564(peroxisomes (leaf))
20706207(cell cycle interactome (cell culture))
19423572(leaf (wt and clpr2-1))
21533090(plasmodesmata-crude)
21325567(root-proteome (wt & det3))
21166475(cytosolic-fractions-crude)
21166475(cytosol-enriched&curated)
20733066(ABA effected phosphoproteome)
20733066(phosphoproteome-ABA-leaf)
21173025(root proteome)
21988472(vacuole (unfiltered data))

Comparative Proteomics Data

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