PPDB

 The Plant Proteome Database

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AT1G12410.1   CLP protease proteolytic subunit 2
Lab Annot. ClpR2 (R-ring)
Mapman: 29.5.5.8.1* protein.degradation.serine protease.SK.S14
Curated Location plastid stroma (Pubmed: 23198870 11278690 18431481 ) (supported by in-house exprements)

TAIR curated location: chloroplastic endopeptidase Clp complex & chloroplast stroma & thylakoid (sensu Viridiplantae)11278690
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
ATCG00670.1 11Arabidopsis thaliana4E-481933862894 (634)_
AT5G23140.1 11Arabidopsis thaliana8E-442163761230 (99)M
AT1G02560.1 11Arabidopsis thaliana4E-4217441641206 (865)C
Os06g04530.1 7Oryza sativa5E-119266608034 (34)C
Osp1g00590.1 7Oryza sativa2E-44195406030 (30)_
Os08g15270.1 7Oryza sativa1E-43195405930 (30)_
v1_GRMZM2G148106_P01 4a53Zea mays5E-121275617856 (56)C
GRMZM2G148106_P01 3.21Zea mays5E-1212756178151 (68)C
Zm00001eb377860_P002 agpv5Zea mays5E-12127561780 (0)C
Show all       

#Spots: The number of publicly accessible spots are in parenthesis
Prediction
PFAM: CLP_protease(1)
TargetP: Chloroplast (Class 3 C0.724; M0.155; S0.036; _0.199)
Predotar:
Subcel. Location: Stroma
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 279 aa (-cTP 225)
Molecular Weight 31.21 kDA(-cTP 25.24)
PI 9.19(-cTP 8.59)

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  
Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
94Detail       root [B. rapa] plastid stroma 
99Detail       petal [B. oleracea] plastid stroma 
119Detail       root [B. rapa] plastid stroma 
120Detail       root [B. rapa] plastid stroma 
127Detail       leaf [A. thaliana] plastid thylakoid membranewild-type
135Detail       leaf [A. thaliana] plastid thylakoid membranewild-type
151Detail       leaf [A. thaliana] chloroplast stromawild-type
180_181Detail       leaf [A. thaliana] chloroplast stromaclpr2-1
187_188Detail       leaf [A. thaliana] chloroplast stromaclpr2-1
195_194Detail       leaf [A. thaliana] chloroplast stromaffc1-2
249_250Detail       leaf [A. thaliana] chloroplast stromaffc1-2
303Detail       leaf [A. thaliana] chloroplast stromawild-type
311Detail       leaf [A. thaliana] total leaf tissue clpr2-1:R2-his6
326Detail       leaf [A. thaliana] chloroplast stromawild-type
339Detail       leaf [A. thaliana] chloroplast stromawild-type
342Detail       leaf [A. thaliana] chloroplast low density membranewild-type
351Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
352Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
355Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
356Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
361Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
371Detail       leaf [A. thaliana] total leaf tissue clpr4-1
411Detail        [A. thaliana] QConCat (E. coli) none (E. coli)
412Detail       leaf [A. thaliana] stroma - R-X102 tag-CNPAGE clpr2-1-TapTagR2
420Detail       leaf [A. thaliana] chloroplast stromaclpR1-stop (EMS)
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
528Detail       leaf [A. thaliana] total leaf tissue wild-type (wt2)
544Detail       leaf [A. thaliana] stroma (FPLC) <600 kDa (LMA) wild-type
563Detail        [A. thaliana]  
617Detail       leaf [A. thaliana] chloroplast stromawild-type
619Detail       leaf [A. thaliana] chloroplast stromawild-type
621Detail       leaf [A. thaliana] total leaf tissue clpr4-1
622Detail       leaf [A. thaliana] total leaf tissue wild-type
646Detail        [A. thaliana] Qconcat  
698Detail       leaf [A. thaliana] stroma- QconCAT wild-type
755Detail        [A. thaliana]  
869Detail       leaf [A. thaliana] chloroplast stromawild-type
870Detail       leaf [A. thaliana] chloroplast stromawild-type
871Detail       leaf [A. thaliana] chloroplast stromawild-type
873Detail       leaf [A. thaliana] chloroplast stromawild-type
874Detail       leaf [A. thaliana] chloroplast stromawild-type
877Detail       leaf [A. thaliana] chloroplast stromawild-type
888Detail        [A. thaliana] R4Strep
1091Detail        [A. thaliana] R4Strep Complemented
1127Detail       leaf [A. thaliana] chloroplast plastoglobulek2k3
1176Detail       leaf [A. thaliana] co-IP ClpR2 stromawild-type
1179Detail       leaf [A. thaliana] co-IP ClpR2 stromaclpr2-1
1197Detail       leaf [A. thaliana] co-IP ClpR2 stromaclpr2-1
1202Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1207Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1208Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1209Detail       leaf [A. thaliana] total leaf tissue StrepII-tagged Clp transgenic
1213Detail       leaf [A. thaliana] total leaf tissue P3-StrepII complemented
1215Detail       leaf [A. thaliana] co-IP ClpR2 stromawild-type
1345Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 1)
1346Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 2)
1347Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 3)
1348Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 1)
1349Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 2)
1350Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 3)
1393Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1394Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1395Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1396Detail       leaf [A. thaliana] total leaf tissue clpt1xclpt2 (rep. 2)
1397Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 3)
1399Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1400Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1402Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1404Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1405Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1406Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1407Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1408Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1409Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 wild-type
1410Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 wild-type
1413Detail       leaf [A. thaliana] total leaf tissue -114 rep1tR1 wild-type
1414Detail       leaf [A. thaliana] total leaf tissue -114 rep1tR2 wild-type
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1425Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 2)
1431Detail       leaf [A. thaliana] total tissue (2% sucrose) wild-type (rep. 2)
1439Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 1)
1440Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 2)
1441Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 3)
1442Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 1)
1443Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 2)
1444Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 3)
1445Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 1)
1446Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 2)
1447Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 3)
1448Detail       leaf [A. thaliana] total tissue (3 day HL) abck2xabck3 (rep. 1)
1450Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 3)
1455Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 1)
1456Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 2)
1457Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 3)
1458Detail       leaf [A. thaliana] chloroplast stromaclps (rep. 1)
1459Detail       leaf [A. thaliana] chloroplast stromaclps (rep. 2)
1460Detail       leaf [A. thaliana] chloroplast stromaclps (rep. 3)
1461Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 1)
1462Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 2)
1463Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 3)
1465Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 1)
1467Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 2)
1481Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 1)
1482Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 1)
1483Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 2)
1484Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 2)
1485Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 3)
1486Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 3)
1517Detail       leaf [A. thaliana] wild-type
1518Detail       leaf [A. thaliana] wild-type
1519Detail       leaf [A. thaliana] wild-type
1520Detail       leaf [A. thaliana] wild-type
1523Detail       leaf [A. thaliana] wild-type
1529Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP wild-type (rep. 1)
1530Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP wild-type (rep. 1)
1532Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP clps (rep. 1)
1533Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP clps (rep. 1)
1534Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP clps (rep. 1)
1542Detail       leaf [A. thaliana] wild-type
1567Detail       leaf [A. thaliana] TiO2 thylakoid membranek2k3
1568Detail       leaf [A. thaliana] TiO2 thylakoid membraneWT
1633Detail       leaf [A. thaliana] Stroma stromawild-type
1634Detail       leaf [A. thaliana] Stroma stromawild-type
1635Detail       leaf [A. thaliana] Stroma stromaclpt1-2
1636Detail       leaf [A. thaliana] Stroma stromaclpt1-2
1637Detail       leaf [A. thaliana] total leaf tissue stromaWild-type
1638Detail       leaf [A. thaliana] total leaf tissue stromaWild-type
1682Detail        [A. thaliana] t1-t2xt2-t1/T1strepII
1683Detail        [A. thaliana] t1-t2xt2-t1/T1strepII
1684Detail        [A. thaliana] t1-t2xt2-t1/T2strepII
1870Detail       Total leaf [A. thaliana] Stroma wild-type
1933Detail       leaf [A. thaliana] chloroplast wild-type
1935Detail       leaf [A. thaliana] chloroplast wild-type
1936Detail       leaf [A. thaliana] chloroplast Prep1_Prep2
1937Detail       leaf [A. thaliana] chloroplast Prep1_Prep2
1941Detail       leaf [A. thaliana] stroma WT stromawild-type
1942Detail       leaf [A. thaliana] stroma WT stromawild-type
1943Detail       leaf [A. thaliana] stroma WT stromawild-type
1944Detail       leaf [A. thaliana] stroma UVR stromaUVR
1945Detail       leaf [A. thaliana] stroma UVR stromaUVR
1946Detail       leaf [A. thaliana] stroma UVR stromawild-type
1947Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1948Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1949Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1985Detail       leaf [A. thaliana] plastid wild-type
1986Detail       leaf [A. thaliana] plastid Prep1_Prep2
1987Detail       leaf [A. thaliana] plastid Prep1_Prep2
1988Detail       leaf [A. thaliana] plastid Prep1_Prep2
1999Detail       leaf [A. thaliana] stroma ClpS stromaclpS1
2000Detail       leaf [A. thaliana] stroma ClpS stromaclpS1
2001Detail       leaf [A. thaliana] stroma ClpS stromaclpS1
2002Detail       leaf [A. thaliana] stroma ClpS stromaclpS1
2007Detail       leaf [A. thaliana] WT PG plastoglobuleWT
2009Detail       leaf [A. thaliana] total leaf tissue wild-type
2010Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2011Detail       leaf [A. thaliana] total leaf tissue opda1-2
2012Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2013Detail       leaf [A. thaliana] total leaf tissue wt
2014Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2015Detail       leaf [A. thaliana] total leaf tissue opda1-2
2016Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2017Detail       leaf [A. thaliana] total leaf tissue wild-type
2018Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2019Detail       leaf [A. thaliana] total leaf tissue opda1-2
2020Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2112Detail       leaf [A. thaliana] Stroma stromawt
2113Detail       leaf [A. thaliana] Stroma stromawt
2114Detail       leaf [A. thaliana] Stroma stromawt
2115Detail       leaf [A. thaliana] Stroma stromacGep
2116Detail       leaf [A. thaliana] Stroma stromacGep
2117Detail       leaf [A. thaliana] Stroma stromacGep
2160Detail       leaf [A. thaliana] stroma Clp stromaP3-strepII 13
2162Detail       leaf [A. thaliana] stroma Clp stromaP3-trap - strepII 8
2163Detail       leaf [A. thaliana] stroma Clp stromaP5-trap - strepII 10
2164Detail       leaf [A. thaliana] stroma Clp stromaP3-strepII
2165Detail       leaf [A. thaliana] stroma Clp stromaP3-trap-strepII
2166Detail       leaf [A. thaliana] stroma Clp stromaP5 trap-strepII
2167Detail       leaf [A. thaliana] stroma Clp stromaP3 trap-strepII
2185Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2190Detail        [A. thaliana] plastid stroma stromaP3-strepII 31
2191Detail        [A. thaliana] plastid stroma stromaP3-trap-strepII 40
2192Detail        [A. thaliana] total soluble P3-trap-strepII 16
2193Detail        [A. thaliana] total soluble P3-strepII 26
2208Detail       leaf [A. thaliana] total leaf tissue Clp P5 strepII
2209Detail       leaf [A. thaliana] total leaf tissue Clp P5 Trapst 10
2210Detail       leaf [A. thaliana] total leaf tissue Clp P5 strepII st28
2211Detail       leaf [A. thaliana] total leaf tissue Clp P5 Trapst 12
2214Detail       leaf [A. thaliana] total leaf tissue Clp P5 st
2215Detail       leaf [A. thaliana] total leaf tissue Clp P5 trap st
2216Detail       leaf [A. thaliana] total leaf tissue Clp P5 st
2217Detail       leaf [A. thaliana] total leaf tissue Clp P5 trap st
2218Detail       Total soluble [A. thaliana] Total soluble proteins cGEP strep
2222Detail       Total soluble [A. thaliana] Total soluble proteins cGEP strep
2223Detail       Total soluble [A. thaliana] Total soluble proteins cGEP strep
2224Detail       leaf [A. thaliana] total leaf tissue Clp P3 strep
2225Detail       leaf [A. thaliana] total leaf tissue Clp P3 trap
2226Detail       leaf [A. thaliana] total leaf tissue wild-type
2245Detail       seedlings [A. thaliana] total tissue wild-type
2247Detail       seedlings [A. thaliana] total tissue atg5
2248Detail       seedlings [A. thaliana] total tissue wild-type
2249Detail       seedlings [A. thaliana] total tissue gfs9
2250Detail       seedlings [A. thaliana] total tissue atg5
2251Detail       seedlings [A. thaliana] total tissue wild-type
2252Detail       seedlings [A. thaliana] total tissue gfs9
2261Detail       leaf [A. thaliana] stroma Clp clp trap
2263Detail       leaf [A. thaliana] stroma Clp Clp C1 trap
2265Detail       leaf [A. thaliana] stroma Clp stromaClp C1 trap
2267Detail       leaf [A. thaliana] stroma Clp Clp C1 TRAP
2285Detail        [A. thaliana] Clp C1 TRAP
2289Detail        [A. thaliana] Clp C1 TRAP
2291Detail        [A. thaliana] Clp C1 TRAP
2296Detail        [A. thaliana] Clp C1 WT
2297Detail        [A. thaliana] Clp C1 WT
2298Detail        [A. thaliana] Clp C1 WT
2302Detail        [A. thaliana] Clp C1 WT
2303Detail        [A. thaliana] Clp C1 WT
2304Detail        [A. thaliana] Clp C1 WT
2305Detail        [A. thaliana] Clp C1 TRAP
2306Detail        [A. thaliana] Clp C1 TRAP
2307Detail        [A. thaliana] Clp C1 TRAP
2400Detail       leaf-soluble [A. thaliana] GFP-nano-trap wt/DUF760-1GFP/ClpC1TRAPSTREP

* For details about the exprimental sources click here.

Published Proteomics Data

 12938931(Total chloroplast envelope)
12766230(Total chloroplast envelope)
16207701(chloroplast stroma)
18538804(total leaf)
17828791(total leaf)
18633119(stroma)
18431481(chloroplast)
19546170(mature pollen grains)
19525416(leaf (wt and clpr4-1 mutant))
20423899(chloroplast)
19329564(peroxisomes (leaf))
20706207(contaminants cell cycle interactome (cell culture))
20061580(chloroplast envelope (inner+outer))
19995723(chloroplast reference proteome)
21311031(chloroplasts)
21173025(root proteome)
21515685(GreenCut2)

Comparative Proteomics Data
Expr*SourceTech.SpotPepSeqChargeState(z)AreaRatioAmbiguityTissueSample from
195_194ffc1-2 cICAT- exp1 / wt cICAT- exp1cICAT5SPVGTHCVGLAYNLAGFLLAAGEK + ICAT_l / SPVGTHCVGLAYNLAGFLLAAGEK + ICAT_h30.87 leaf [A. thaliana] chloroplast stroma

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