PPDB

 The Plant Proteome Database

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AT1G03310.2   debranching enzyme 1
Lab Annot. isoamylase 2 (lSA2 or DBE1)
Mapman: 2.1.2.4 major CHO metabolism.synthesis.starch.debranching
Curated Location plastid (Pubmed: 23198870 9761796 18431481 ) (supported by in-house exprements)
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT1G03310.1 11Arabidopsis thaliana0882100100172 (68)C
AT4G09020.1 11Arabidopsis thaliana8E-1127133550265 (129)C
AT2G39930.1 11Arabidopsis thaliana3E-926963147154 (59)C
Os05g32710.1 7Oryza sativa073050660 (0)C
Os08g40930.1 7Oryza sativa1E-9969033480 (0)C
Os09g29404.1 7Oryza sativa4E-5839634500 (0)_
Zm00001eb287400_P001 agpv5Zea mays071653680 (0)C
GRMZM2G090905_P01 3.21Zea mays0631556929 (12)C
v1_GRMZM2G090905_P01 4a53Zea mays0631556910 (10)C
Show all       

#Spots: The number of publicly accessible spots are in parenthesis
Prediction
PFAM: Alpha-amylase(3) CBM_48(1)
TargetP: Chloroplast (Class 2 C0.859; M0.069; S0.017; _0.116)
Predotar:
Subcel. Location: Stroma
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 882 aa (-cTP 855)
Molecular Weight 98.88 kDA(-cTP 96.02)
PI 5.65(-cTP 5.54)

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  
Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
303Detail       leaf [A. thaliana] chloroplast stromawild-type
339Detail       leaf [A. thaliana] chloroplast stromawild-type
342Detail       leaf [A. thaliana] chloroplast low density membranewild-type
542Detail       leaf [A. thaliana] stroma (FPLC) >500 kDa (HMB) wild-type
543Detail       leaf [A. thaliana] stroma (FPLC) >600 kDa (HMA) wild-type
622Detail       leaf [A. thaliana] total leaf tissue wild-type
698Detail       leaf [A. thaliana] stroma- QconCAT wild-type
755Detail        [A. thaliana]  
869Detail       leaf [A. thaliana] chloroplast stromawild-type
870Detail       leaf [A. thaliana] chloroplast stromawild-type
871Detail       leaf [A. thaliana] chloroplast stromawild-type
874Detail       leaf [A. thaliana] chloroplast stromawild-type
877Detail       leaf [A. thaliana] chloroplast stromawild-type
1202Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1207Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1208Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1423Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 3)
1460Detail       leaf [A. thaliana] chloroplast stromaclps (rep. 3)
1461Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 1)
1467Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 2)
1481Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 1)
1482Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 1)
1483Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 2)
1484Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 2)
1485Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 3)
1486Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 3)
1651Detail       leaf [A. thaliana] plastoglobules plastoglobulewild-type
1933Detail       leaf [A. thaliana] chloroplast wild-type
1936Detail       leaf [A. thaliana] chloroplast Prep1_Prep2
1937Detail       leaf [A. thaliana] chloroplast Prep1_Prep2
1938Detail       leaf [A. thaliana] chloroplast Prep1_Prep2
1947Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1948Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1949Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1983Detail       leaf [A. thaliana] plastid wild-type
1984Detail       leaf [A. thaliana] plastid wild-type
1985Detail       leaf [A. thaliana] plastid wild-type
1986Detail       leaf [A. thaliana] plastid Prep1_Prep2
1987Detail       leaf [A. thaliana] plastid Prep1_Prep2
1988Detail       leaf [A. thaliana] plastid Prep1_Prep2
1999Detail       leaf [A. thaliana] stroma ClpS stromaclpS1
2112Detail       leaf [A. thaliana] Stroma stromawt
2113Detail       leaf [A. thaliana] Stroma stromawt
2114Detail       leaf [A. thaliana] Stroma stromawt
2115Detail       leaf [A. thaliana] Stroma stromacGep
2116Detail       leaf [A. thaliana] Stroma stromacGep
2117Detail       leaf [A. thaliana] Stroma stromacGep
2179Detail       leaf [A. thaliana] stroma Clp GST ClpF x ClpS1

* For details about the exprimental sources click here.

Published Proteomics Data

 18431481(chloroplast)
20423899(chloroplast)
19995723(chloroplast reference proteome)

Comparative Proteomics Data

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