PPDB

PPDB	The Plant Proteome Database

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AT1G05140.1 Peptidase M50 family protein

Lab Annot.

membrane zinc metalloprotease (RIP)
Mapman: 29.5.7.10.1* protein.degradation.metalloprotease.MM.M50

Curated Location

plastid (Pubmed: 23198870 )

Species

Arabidopsis thaliana Source: TAIR Arabidopsis (v.11)

Links

TAIR PeptideAtlas POGS SUBA Uniprot PTM Get sequence

Related Genes

Acc ver Species Evalue Matchlen %Iden %Similar #spots TargetP
AT2G32480.1 11 Arabidopsis thaliana 0 450 80 87 16 (14) C
AT2G32480.2 11 Arabidopsis thaliana 0 450 74 80 14 (12) C
Os03g38230.1 7 Oryza sativa 1E-149 393 58 75 24 (24) C
Os07g48440.1 7 Oryza sativa 6E-18 73 54 78 0 (0) _
Os02g32960.1 7 Oryza sativa 3E-11 77 57 79 0 (0) _
Zm00001eb219720_P001 agpv5 Zea mays 4E-151 419 57 72 0 (0) C
v1_GRMZM2G062470_P01 4a53 Zea mays 4E-151 419 57 72 50 (50) C
GRMZM2G062470_P01 3.21 Zea mays 4E-151 419 57 72 60 (60) C
Show all

#Spots: The number of publicly accessible spots are in parenthesis

Prediction

PFAM:	PDZ(1) PDZ_2(1) PDZ_6(1) Peptidase_M50(1) Peptidase_M50B(1)
TargetP:	Chloroplast (Class 1 C0.955; M0.016; S0.028; _0.119)
Predotar:
Subcel. Location:	Thylakoid membrane
TM-HMM prediction:	3 TMs
Aramemnon:	4 TMs
TAT position:
Length	441 aa (-cTP 357) (-lTP 354)
Molecular Weight	47.77 kDA(-cTP 38.21)(-lTP 37.98)
PI	9.06(-cTP 5.75)(-lTP 5.757)

Experimental Evidence

Expand

Expr*	Spot	MW native	MW denatured	pI native	pI denatured	Type	Mowse	Ambiguity	Tissue	Sample from	Genotype
343	Detail								leaf [A. thaliana]	chloroplast thylakoid membrane	wild-type
876	Detail								leaf [A. thaliana]	chloroplast thylakoid membrane	wild-type
1441	Detail								leaf [A. thaliana]	total tissue (0 day HL)	wild-type (rep. 3)
1443	Detail								leaf [A. thaliana]	total tissue (0 day HL)	abck2xabck3 (rep. 2)
1643	Detail								leaf [A. thaliana]	total leaf tissue	Wild-type
1708	Detail								[A. thaliana]		Wild-type
1830	Detail								leaf [A. thaliana]	Thylakoids thylakoid membrane	Col-0 Normal light
1983	Detail								leaf [A. thaliana]	plastid	wild-type
1984	Detail								leaf [A. thaliana]	plastid	wild-type
1985	Detail								leaf [A. thaliana]	plastid	wild-type
2320	Detail								[A. thaliana]	Thylakoids plastoglobule
2321	Detail								leaf [A. thaliana]
2324	Detail								leaf [A. thaliana]	THYLAKOIDS thylakoid membrane
2325	Detail								leaf [A. thaliana]	thylakoids thylakoid membrane	abc1k6

* For details about the exprimental sources click here.

Published Proteomics Data

18431481(chloroplast)
19334764(plasma membrane (cell culture))
21189294(nuclear pore complex (co-IPs RAE1,Nup43,50a,93a,136))

Comparative Proteomics Data