PPDB

 The Plant Proteome Database

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AT1G02500.1   S-adenosylmethionine synthetase 1
Lab Annot. S-adenosylmethionine synthetase 1 (SAM1)
Mapman: 13.1.3.4.11 amino acid metabolism.synthesis.aspartate family.methionine.S-adenosylmethionine synthetase
Curated Location not plastid (supported by in-house exprements)
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT1G02500.2 11Arabidopsis thaliana0393100100319 (154)_
AT4G01850.1 11Arabidopsis thaliana03939698330 (138)_
AT4G01850.2 11Arabidopsis thaliana03939698327 (135)_
Os01g22010.2 7Oryza sativa0392929649 (49)_
Os01g22010.3 7Oryza sativa0392929649 (49)_
Os01g22010.1 7Oryza sativa0392929649 (49)_
GRMZM2G117198_P02 3.21Zea mays0393919665 (52)S
v1_GRMZM2G117198_P02 4a53Zea mays0393919691 (90)S
GRMZM2G117198_P01 3.21Zea mays0393919673 (60)_
Show all       

#Spots: The number of publicly accessible spots are in parenthesis
Prediction
PFAM: MSA_2(1) S-AdoMet_synt_C(2) S-AdoMet_synt_M(1) S-AdoMet_synt_N(1)
TargetP: Cytoplasm (Class 2 C0.128; M0.108; S0.154; _0.870)
Predotar:
Subcel. Location:
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 393 aa (-cTP 374)
Molecular Weight 43.16 kDA(-cTP )
PI 5.51(-cTP )

Experimental Evidence

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View Identified Peptides  

View GeneModel  
Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
351Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
355Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
371Detail       leaf [A. thaliana] total leaf tissue clpr4-1
528Detail       leaf [A. thaliana] total leaf tissue wild-type (wt2)
621Detail       leaf [A. thaliana] total leaf tissue clpr4-1
622Detail       leaf [A. thaliana] total leaf tissue wild-type
1113Detail        [A. thaliana] control for RARE-1 co-IP wild-type
1127Detail       leaf [A. thaliana] chloroplast plastoglobulek2k3
1392Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1393Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1394Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1395Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1396Detail       leaf [A. thaliana] total leaf tissue clpt1xclpt2 (rep. 2)
1400Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1402Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1404Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1405Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1406Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1407Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1408Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1409Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 wild-type
1411Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 clpr2-1
1412Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 clpr2-1
1413Detail       leaf [A. thaliana] total leaf tissue -114 rep1tR1 wild-type
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1431Detail       leaf [A. thaliana] total tissue (2% sucrose) wild-type (rep. 2)
1439Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 1)
1444Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 3)
1445Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 1)
1447Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 3)
1448Detail       leaf [A. thaliana] total tissue (3 day HL) abck2xabck3 (rep. 1)
1518Detail       leaf [A. thaliana] wild-type
1519Detail       leaf [A. thaliana] wild-type
1520Detail       leaf [A. thaliana] wild-type
1533Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP clps (rep. 1)
1534Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP clps (rep. 1)
1596Detail        [A. thaliana]  
1749Detail       leaf [A. thaliana] total leaf tissue DXS_Flag
1756Detail       leaf [A. thaliana] total leaf tissue Col_0
1758Detail       leaf [A. thaliana] total leaf tissue DXS_Flag+fosmidomycin
1874Detail       Total leaf [A. thaliana] total soluble wild-type
1875Detail       Total leaf [A. thaliana] total soluble wild-type
1876Detail       Total leaf [A. thaliana] total soluble wild-type
1930Detail       leaf [A. thaliana] total leaf tissue Col_0 Wild-type
1931Detail       leaf [A. thaliana] total leaf tissue DXS_Flag
2009Detail       leaf [A. thaliana] total leaf tissue wild-type
2011Detail       leaf [A. thaliana] total leaf tissue opda1-2
2012Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2013Detail       leaf [A. thaliana] total leaf tissue wt
2014Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2015Detail       leaf [A. thaliana] total leaf tissue opda1-2
2016Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2017Detail       leaf [A. thaliana] total leaf tissue wild-type
2018Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2019Detail       leaf [A. thaliana] total leaf tissue opda1-2
2020Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2030Detail       leaf [A. thaliana] over expressed M48 plastoglobuleM48
2182Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2183Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2184Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2185Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2193Detail        [A. thaliana] total soluble P3-strepII 26
2215Detail       leaf [A. thaliana] total leaf tissue Clp P5 trap st
2224Detail       leaf [A. thaliana] total leaf tissue Clp P3 strep
2245Detail       seedlings [A. thaliana] total tissue wild-type
2247Detail       seedlings [A. thaliana] total tissue atg5
2248Detail       seedlings [A. thaliana] total tissue wild-type
2250Detail       seedlings [A. thaliana] total tissue atg5
2252Detail       seedlings [A. thaliana] total tissue gfs9
2255Detail       leaf [A. thaliana] total seedlings gfs9
2256Detail       leaf [A. thaliana] total seedlings gfs9
2286Detail        [A. thaliana] Clp C1 WT
2287Detail        [A. thaliana] Clp C1 WT
2288Detail        [A. thaliana] Clp C1 WT
2289Detail        [A. thaliana] Clp C1 TRAP
2290Detail        [A. thaliana] Clp C1 TRAP
2291Detail        [A. thaliana] Clp C1 TRAP
2296Detail        [A. thaliana] Clp C1 WT
2297Detail        [A. thaliana] Clp C1 WT
2298Detail        [A. thaliana] Clp C1 WT
2299Detail        [A. thaliana] Clp C1 TRAP
2300Detail        [A. thaliana] Clp C1 TRAP
2301Detail        [A. thaliana] Clp C1 TRAP
2303Detail        [A. thaliana] Clp C1 WT
2304Detail        [A. thaliana] Clp C1 WT
2306Detail        [A. thaliana] Clp C1 TRAP
2307Detail        [A. thaliana] Clp C1 TRAP
2308Detail       leaf [A. thaliana] PG plastoglobule 
2310Detail       leaf [A. thaliana] PG plastoglobule 
2316Detail       leaf [A. thaliana] PG plastoglobule 
2317Detail       leaf [A. thaliana] PG plastoglobule 
2318Detail       leaf [A. thaliana] PG plastoglobule 
2398Detail       leaf-soluble [A. thaliana] GFP-nano-trap wt/35SRecA-GFP
2399Detail       leaf-soluble [A. thaliana] GFP-nano-trap wt/DUF760-1GFP/ClpC1WTSTREP
2400Detail       leaf-soluble [A. thaliana] GFP-nano-trap wt/DUF760-1GFP/ClpC1TRAPSTREP

* For details about the exprimental sources click here.

Published Proteomics Data

 16287169(Cell Wall proteome)
16242667(mature Pollen)
16358359(cell suspension (Gamborg))
16358359(cell suspension (M&Skoog))
16502469(cell suspension-Cd-up)
15815986(seedling)
17317660(Plasma Membrane proteome)
18538804(total leaf)
17916636(root (NaCl stress))
17432890(leaf total membranes)
18814325(cotyledons)
19200160(flower-stage 12)
19546170(mature pollen grains)
20118269(developing seeds)
19525416(leaf (wt and clpr4-1 mutant))
17216043(leaf)
19334764(plasma membrane (cell culture))
19888209(80S polysomal fraction)
19888209(leaves)
20706207(contaminants cell cycle interactome (cell culture))
18445580(leaves)
20166762(guard cells (Arabidopsis))
19114538(guard cells Arabidopsis leaf)
19508356(mature pollen grains and pollen tubes)
19423572(leaf (wt and clpr2-1))
19292762(Ubiquitin-affinity seedling (10 d))
21325567(root-proteome (wt & det3))
21166475(cytosolic-fractions-crude)
21166475(cytosol-enriched&curated)
21173025(root proteome)

Comparative Proteomics Data

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